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Image Search Results
Journal: Science signaling
Article Title: ELP-dependent expression of Mcl-1 promotes resistance to EGFR inhibition in triple-negative breast cancer cells #
doi: 10.1126/scisignal.abb9820
Figure Lengend Snippet: (A) Lethal fraction kinetics following application of 10 μM erlotinib. Cells tested were BT20 + scrambled RNA (SCX), or BT20 + siRNA targeting ELP3, 4, 5, or 6 (ELP KD). Data are mean +/− SD from 3 biological replicate wells. Death rates for each ELP KD compared to SCX (Dunnett’s test). (B-C) CellTrace proliferation dye dilution to determine growth rate of live cells. (B) BT20 cells with scrambled RNA or ELP4 targeted siRNA, untreated (Unt) or given 10 μM erlotinib (ERL). Samples collected prior to or 72 hours after drug application. Representative dye fluorescence distributions from flow cytometry shown based on 3 biological replicate samples with at least 10,000 cells measured per sample. (C) Quantification of cell growth rate from data in (B). Data are mean +/− SD of 3 biological replicate samples. (D) Mcl-1 protein expression in BT20 cells + SCX or + ELP4 siRNA, with or without 10 μM ERL. Blots are representative of 3 biological replicates which all showed similar results. (E) Proteome profiler apoptotic array. Data shown are representative of 3 biological replicate blots from ELP4 siRNA untreated or ELP4 siRNA + 10 μM ERL. (F) Quantification of (D) and (E). Data are mean +/− SD of 3 biological replicate blots. Labelled signals are significantly different in ELP4 KD cells compared to Scrambled RNA cells (t-test with FDR correction). See also figs. S6–S9. For panels (A), (C), and (F): *p < 0.05; **p < 0.01; ***p < 0.001; n.s., not significant.
Article Snippet:
Techniques: Fluorescence, Flow Cytometry, Expressing